During testing, the specimen is added to the sample well (S) and reacts with anti-chlamydia trachomatics antibodies conjugated to colored particles and precoated onto the sample pad of the test. Then, the mixture migrates through the membrane by capillary action and interacts with reagents on the membrane. If there is sufficient C. trachomatics antigens in the specimen, a colored band will form at the test well (T) of the membrane. The presence of this colored band indicates a positive result, while its absence indicates a negative result. The appearance of a colored band at the control well serves as a procedural control, indicating that the  proper  volume  of  specimen  has  been  added  and  membrane  wicking  has occurred.