Mycoplasma is one of the main pathogens which lead to nongonoccal urethritis (NGU), cervical, pelvic inflammatory disease, such as orchitis, epididymitis etc, and cause infertility to men and women. These pathogens can attack and destroy urogenital epithelial cells, causing infection of AIDS and other sexually transmitted diseases. 

Among all the Mycoplasma species, UU and MH are able to lead sexually transmitted diseases. The incidence is continuously increasing, seriously compromising human health. The key to the treatment and prevention of the spread of Mycoplasma is timely and accurate detection. Currently, culture method is still being recognized as a reliable method to identify Mycoplasma infection.